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antibodies slc3a2  (Proteintech)


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    Structured Review

    Proteintech antibodies slc3a2
    Antibodies Slc3a2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slc3a2+antibody/CD98%2FSLC3A2+Antibody/10__1007_slash_s13402___026___01299___y-159-10-13
    Average 96 stars, based on 132 article reviews
    antibodies slc3a2 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Knockdown:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Western Blot:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Quantitative RT-PCR:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Over Expression:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Binding Assay:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Modification:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    ChIP-qPCR:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Mutagenesis:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Clone Assay:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Luciferase:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Plasmid Preparation:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Activity Assay:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Transfection:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Control:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Stable Transfection:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    CCK-8 Assay:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Colony Assay:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Transwell Assay:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Migration:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Injection:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Membrane:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Immunohistochemistry:

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis.
    Article Snippet: Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HAtag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).

    Article Title: JMJD6 K375 acetylation restrains lung cancer progression by enhancing METTL14/m6A/SLC3A2 axis mediated cell ferroptosis
    Article Snippet: Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.Both DMED and RPMI-1640 were supplemented with 10% (vol/vol) fetal bovine serum (FBS) and 1% penicillin/streptomycin.. The antibodies used in this study were listed as follows: JMJD6 antibody (Santa Cruz Biotechnology, Cat# sc-28348, 1:1000), METTL14 antibody (CST, Cat# 48699, 1:1000), SLC3A2 antibody (Abways, Cat# CY8416, 1:1000), GAPDH antibody (Abways, Cat# AB2000, 1:6000), Flag-tag antibody (Abways, Cat# AB0030, 1:1000), HA-tag antibody (proteintech, Cat# 51064-2-AP, 1:3000), Acetylated-Lysine Antibody (CST, Cat# 9441, 1:1000), JMJD6-acK375 antibody (Jiaxuan Biotech Cat#JXR7611, 1:1000), Rabbit Anti-Goat IgG (H + L) HRP (Abways, Cat# AB0101, 1:8000).



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    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of <t>SLC3A2</t> and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
    Antibodies Targeting Slc3a2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of <t>SLC3A2</t> and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
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    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Cell Reports Medicine

    Article Title: Multi-omics profiling unveils biological and clinical insights into pulmonary sarcomatoid carcinoma

    doi: 10.1016/j.xcrm.2026.102887

    Figure Lengend Snippet: Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: Cells were lysed with 1% Triton X-100 buffer (Beyotime, ST1723) and then boiled in 5× loading buffer for 10 min. Each sample was electrophoresed on 10% PAGE gels, then transferred to PVDF membranes and immunoblotted with the antibodies targeting SLC3A2 (Abclonal, A24375), FTL (Abclonal, A11241), GPX4 (Abclonal, A1933) and β-Actin (Abclonal, AC004).

    Techniques: Western Blot, Knockdown, CCK-8 Assay, Cell Culture, Staining, Software, Concentration Assay, Acid Assay, Negative Control